Cell-Wall Lipopolysaccharide of Escherichia coli O114:H2. Structure of the Polysaccharide Chain
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چکیده
منابع مشابه
The Biosynthesis of Cell Wall Lipopolysaccharide in Escherichia coli
The cell wall lipopolysaccharide of Escherichia coli 011 l-B4 was degraded by (a) acid hydrolysis and (b) by treatment with periodate, followed by reduction with sodium borohydride and mild acid hydrolysis. The various oligosaccharides produced by these procedures were isolated and characterized by chemical and enzymatic methods. Procedure (I released all of the colitose (3,6-dideoxy-~-xylohexo...
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The ultrastructure of the purified and lyophilized endotoxin from Escherichia coli O111 was observed by ultrathin sectioning. Onion-like globular membrane structures were observed in addition to rod-like and ribbon-like structures, indicating the existence of a globular membrane structure even in the dried state.
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Incubation of UDP-N-acetyl[3H]glucosamine with purified inner membrane preparations of a mutant (UDPgalactose-4-epimeraseless and glucosamine-6-phosphate synthetaseless) Escherichiu coli 0111-B4 results in the incorporation of N-acetyl[3H]glucosamine into two components, A and B, that may be distinguished by polyacrylamide gel electrophoresis in the presence of sodium dodecyl sulfate. A contain...
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15 صفحه اولConvergent synthesis of the tetrasaccharide repeating unit of the cell wall lipopolysaccharide of Escherichia coli O40
A tetrasaccharide repeating unit corresponding to the cell-wall lipopolysaccharide of E. coli O40 was synthesized by using a convergent block glycosylation strategy. A disaccharide donor was coupled to a disaccharide acceptor by a stereoselective glycosylation. A 2-aminoethyl linker was chosen as the anomeric protecting group at the reducing end of the tetrasaccharide. All glycosylation steps a...
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ژورنال
عنوان ژورنال: European Journal of Biochemistry
سال: 1983
ISSN: 0014-2956,1432-1033
DOI: 10.1111/j.1432-1033.1983.tb07597.x